Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.
Identifieur interne : 001C97 ( Main/Exploration ); précédent : 001C96; suivant : 001C98Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.
Auteurs : Marja Ilmén [Finlande] ; Merja Oja [Finlande] ; Anne Huuskonen [Finlande] ; Sangmin Lee [Corée du Sud] ; Laura Ruohonen [Finlande] ; Simon Jung [Corée du Sud]Source :
- Metabolic engineering [ 1096-7184 ] ; 2015.
Descripteurs français
- KwdFr :
- Alkyl et aryl transferases (composition chimique), Alkyl et aryl transferases (isolement et purification), Alkyl et aryl transferases (physiologie), Butadiènes (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Génome bactérien (MeSH), Hémiterpènes (biosynthèse), Pentanes (MeSH), Similitude de séquences (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- biosynthèse : Hémiterpènes.
- composition chimique : Alkyl et aryl transferases.
- génétique : Escherichia coli.
- isolement et purification : Alkyl et aryl transferases.
- physiologie : Alkyl et aryl transferases.
- Butadiènes, Données de séquences moléculaires, Génome bactérien, Pentanes, Similitude de séquences, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Alkyl and Aryl Transferases (chemistry), Alkyl and Aryl Transferases (isolation & purification), Alkyl and Aryl Transferases (physiology), Amino Acid Sequence (MeSH), Butadienes (MeSH), Escherichia coli (genetics), Genome, Bacterial (MeSH), Hemiterpenes (biosynthesis), Molecular Sequence Data (MeSH), Pentanes (MeSH), Sequence Homology (MeSH).
- MESH :
- chemical , biosynthesis : Hemiterpenes.
- chemical , chemistry : Alkyl and Aryl Transferases.
- chemical , isolation & purification : Alkyl and Aryl Transferases.
- chemical , physiology : Alkyl and Aryl Transferases.
- genetics : Escherichia coli.
- Amino Acid Sequence, Butadienes, Genome, Bacterial, Molecular Sequence Data, Pentanes, Sequence Homology.
Abstract
Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls.
DOI: 10.1016/j.ymben.2015.08.001
PubMed: 26275749
Affiliations:
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Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo</wicri:regionArea><wicri:noRegion>Espoo</wicri:noRegion></affiliation></author><author><name sortKey="Huuskonen, Anne" sort="Huuskonen, Anne" uniqKey="Huuskonen A" first="Anne" last="Huuskonen">Anne Huuskonen</name><affiliation wicri:level="1"><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. 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Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo</wicri:regionArea><wicri:noRegion>Espoo</wicri:noRegion></affiliation></author><author><name sortKey="Oja, Merja" sort="Oja, Merja" uniqKey="Oja M" first="Merja" last="Oja">Merja Oja</name><affiliation wicri:level="1"><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo</wicri:regionArea><wicri:noRegion>Espoo</wicri:noRegion></affiliation></author><author><name sortKey="Huuskonen, Anne" sort="Huuskonen, Anne" uniqKey="Huuskonen A" first="Anne" last="Huuskonen">Anne Huuskonen</name><affiliation wicri:level="1"><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo</wicri:regionArea><wicri:noRegion>Espoo</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sangmin" sort="Lee, Sangmin" uniqKey="Lee S" first="Sangmin" last="Lee">Sangmin Lee</name><affiliation wicri:level="1"><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Global Technology, SK Innovation, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Ruohonen, Laura" sort="Ruohonen, Laura" uniqKey="Ruohonen L" first="Laura" last="Ruohonen">Laura Ruohonen</name><affiliation wicri:level="1"><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo</wicri:regionArea><wicri:noRegion>Espoo</wicri:noRegion></affiliation></author><author><name sortKey="Jung, Simon" sort="Jung, Simon" uniqKey="Jung S" first="Simon" last="Jung">Simon Jung</name><affiliation wicri:level="1"><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea. Electronic address: jungmoongeun@sk.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Global Technology, SK Innovation, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author></analytic><series><title level="j">Metabolic engineering</title><idno type="eISSN">1096-7184</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (chemistry)</term><term>Alkyl and Aryl Transferases (isolation & purification)</term><term>Alkyl and Aryl Transferases (physiology)</term><term>Amino Acid Sequence (MeSH)</term><term>Butadienes (MeSH)</term><term>Escherichia coli (genetics)</term><term>Genome, Bacterial (MeSH)</term><term>Hemiterpenes (biosynthesis)</term><term>Molecular Sequence Data (MeSH)</term><term>Pentanes (MeSH)</term><term>Sequence Homology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkyl et aryl transferases (composition chimique)</term><term>Alkyl et aryl transferases (isolement et purification)</term><term>Alkyl et aryl transferases (physiologie)</term><term>Butadiènes (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Génome bactérien (MeSH)</term><term>Hémiterpènes (biosynthèse)</term><term>Pentanes (MeSH)</term><term>Similitude de séquences (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Hemiterpenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Hémiterpènes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alkyl et aryl transferases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Alkyl et aryl transferases</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Alkyl et aryl transferases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Butadienes</term><term>Genome, Bacterial</term><term>Molecular Sequence Data</term><term>Pentanes</term><term>Sequence Homology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Butadiènes</term><term>Données de séquences moléculaires</term><term>Génome bactérien</term><term>Pentanes</term><term>Similitude de séquences</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26275749</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-7184</ISSN><JournalIssue CitedMedium="Internet"><Volume>31</Volume><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Metabolic engineering</Title><ISOAbbreviation>Metab Eng</ISOAbbreviation></Journal><ArticleTitle>Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.</ArticleTitle><Pagination><MedlinePgn>153-62</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ymben.2015.08.001</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1096-7176(15)00098-1</ELocationID><Abstract><AbstractText>Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls. </AbstractText><CopyrightInformation>Copyright © 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ilmén</LastName><ForeName>Marja</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oja</LastName><ForeName>Merja</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huuskonen</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sangmin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruohonen</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea. Electronic address: jungmoongeun@sk.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Belgium</Country><MedlineTA>Metab Eng</MedlineTA><NlmUniqueID>9815657</NlmUniqueID><ISSNLinking>1096-7176</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.-</RegistryNumber><NameOfSubstance UI="D019883">Alkyl and Aryl Transferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.-</RegistryNumber><NameOfSubstance UI="C093854">isoprene synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019883" MajorTopicYN="N">Alkyl and Aryl Transferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016680" MajorTopicYN="N">Genome, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010420" MajorTopicYN="N">Pentanes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017385" MajorTopicYN="N">Sequence Homology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Genome mining</Keyword><Keyword MajorTopicYN="N">Homology-based screening</Keyword><Keyword MajorTopicYN="N">Isoprenoid</Keyword><Keyword MajorTopicYN="N">Terpene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>08</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26275749</ArticleId><ArticleId IdType="pii">S1096-7176(15)00098-1</ArticleId><ArticleId IdType="doi">10.1016/j.ymben.2015.08.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Ilmen, Marja" sort="Ilmen, Marja" uniqKey="Ilmen M" first="Marja" last="Ilmén">Marja Ilmén</name></noRegion><name sortKey="Huuskonen, Anne" sort="Huuskonen, Anne" uniqKey="Huuskonen A" first="Anne" last="Huuskonen">Anne Huuskonen</name><name sortKey="Oja, Merja" sort="Oja, Merja" uniqKey="Oja M" first="Merja" last="Oja">Merja Oja</name><name sortKey="Ruohonen, Laura" sort="Ruohonen, Laura" uniqKey="Ruohonen L" first="Laura" last="Ruohonen">Laura Ruohonen</name></country><country name="Corée du Sud"><noRegion><name sortKey="Lee, Sangmin" sort="Lee, Sangmin" uniqKey="Lee S" first="Sangmin" last="Lee">Sangmin Lee</name></noRegion><name sortKey="Jung, Simon" sort="Jung, Simon" uniqKey="Jung S" first="Simon" last="Jung">Simon Jung</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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